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prolong gold antifade solution containing dapi  (Thermo Fisher)


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    Thermo Fisher prolong gold antifade solution containing dapi
    Prolong Gold Antifade Solution Containing Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prolong gold antifade solution containing dapi/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    prolong gold antifade solution containing dapi - by Bioz Stars, 2026-04
    90/100 stars

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    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI)</t> (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.
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    https://www.bioz.com/result/prolong gold antifade solution containing 4′,6-diamidino-2-phenylindole (dapi/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    prolong gold antifade solution containing 4′,6-diamidino-2-phenylindole (dapi - by Bioz Stars, 2026-04
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    prolong gold antifade mounting solution containing dapi  (Thermo Fisher)
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    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI)</t> (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.
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    prolong gold antifade mountant with 4',6-diamidino-2-phenylindole (dapi)–containing mounting solution  (Thermo Fisher)
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    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI)</t> (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.
    Prolong Gold Antifade Mountant With 4',6 Diamidino 2 Phenylindole (Dapi)–Containing Mounting Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prolong gold antifade mountant with 4',6-diamidino-2-phenylindole (dapi)–containing mounting solution/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    prolong gold antifade mountant with 4',6-diamidino-2-phenylindole (dapi)–containing mounting solution - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.

    Journal: Frontiers in Immunology

    Article Title: Circulatory HMGB1 is an early predictive and prognostic biomarker of ARDS and mortality in a swine model of polytrauma

    doi: 10.3389/fimmu.2023.1227751

    Figure Lengend Snippet: HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.

    Article Snippet: After washing, sections were mounted with ProLong Gold Antifade solution containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA) for staining the nuclear DNA.

    Techniques: Immunohistochemistry, Staining, Immunofluorescence, Software, MANN-WHITNEY